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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is observed in many tumors, which leads to chromosome instability, and therefore may perhaps add to tumorigenesis. This protein was uncovered to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle regulated histone gene expression and performs a essential purpose in endorsing cell-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are already explained. Two supplemental splice variants had been claimed but detailed nucleotide sequence facts is just not still obtainable.
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Blocking peptides are peptides that bind especially to your goal antibody and block antibody binding. These peptide usually has the epitope recognized through the antibody.
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This manual outlines further more attainable optimization of mobile and immunoassay parameters to be sure the best possible outcomes are...
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This guideline outlines further more achievable optimization of cellular and immunoassay parameters to be sure the absolute best benefits are...
Antibodies certain to the blocking peptide no more bind on the epitope to the focus on protein. By comparing the staining from your blocked antibody compared to the antibody alone, one can see which staining is unique.
Focus on Details Cyclin E belongs on the really conserved cyclin spouse and children, whose users are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins functionality as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation patterns which add to your temporal coordination of each mitotic function. Cyclin E sorts a posh with and capabilities as a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S stage boundary and is particularly degraded as cells development via S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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